Skip to main content
. 2016 Sep 19;2016:2545214. doi: 10.1155/2016/2545214

Figure 2.

Figure 2

Sema3A promotes osteogenic differentiation while inhibiting adipogenic differentiation in ASCs. (a) ASCs were infected with either empty pWPI or Sema3A overexpression lentivirus, respectively. ASC-Sema3A showed higher ALP activity than ASC-control after 7-day osteogenic induction. (b) Quantification data of ALP activity. (c) Significantly more mineralization nodules were found in ASC-Sema3A group than that in ASC-control group after 21 days of osteogenic induction. (d) Quantification data of ECM mineralization in Figure 2(c). (e) Sema3A significantly reduced lipid droplet formation in ASCs after 9 days of adipogenic induction. (f) Quantification data of lipid droplets in Figure 2(e). (g, h, and i) Expression of the osteogenesis related genes including ALP, RUNX2, and COL-1 after 5 and 10 days of osteogenic induction. (j, k, and l) Expression of the adipogenesis related genes including FABP4, PPARG, and C/EBPA after 3 and 5 days of adipogenic induction. Mean ± SD, n = 3, and ∗ p < 0.05.