Figure 2.

PLD2 is mainly expressed in neutrophils. (a) Expression of PLD2 in different subsets of immune cells. Peripheral neutrophils, CD20⁺ B cells, CD8⁺ T cells, CD4⁺ T cells, and CD14⁺ monocytes (1 × 10⁶) were isolated from healthy donors (n = 10), and expression of PLD2 was detected by qRT-PCR. ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001 versus CD14⁺ monocytes. (b) Neutrophils were isolated from peripheral blood of patients with A-CD (n = 26), R-CD (n = 23), A-UC (n = 26), R-UC (n = 19), and HC (n = 20). Expression of PLD2 mRNA was examined by qRT-PCR. ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001 versus HC. Gene expression was normalized to GAPDH in each group.