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. 2016 Sep 18;2016:2543070. doi: 10.1155/2016/2543070

Figure 3.

Figure 3

TNF-α upregulates PLD2 expression in neutrophils. (a) Neutrophils isolated from healthy donors (n = 10) were stimulated with IL-6 (10 ng/mL), IL-17A (10 ng/mL), TNF-α (10 ng/mL), IFN-γ (10 ng/mL), LPS (10 ng/mL), and IL-1β (10 ng/mL), respectively, for 4 h. Expression of PLD2 mRNA was detected by qRT-PCR. ∗ p < 0.05, ∗∗ p < 0.001, and ∗∗∗ p < 0.01 versus medium alone. (b) Neutrophils isolated from healthy donors (n = 10) were stimulated in vitro with different concentrations of TNF-α as indicated for 4 h; expression of PLD2 was detected by qRT-PCR. ∗ p < 0.05,  ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus that under culture in medium alone. (c) Neutrophils isolated from healthy donors (n = 10) were stimulated with TNF-α (10 ng/mL) for 2, 4, and 6 h, and expression of PLD2 was detected by qRT-PCR. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus that at the beginning of stimulation. Gene expression was normalized to GAPDH in each group.