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. 2016 Aug 9;149(3):320–328. doi: 10.1111/imm.12647

Figure 3.

Figure 3

The expression of inflammatory cytokines in peritoneal M1‐ and M2‐polarized macrophages. Total RNA was isolated from the M1‐ and M2‐polarized macrophages after stimulation without lipopolysaccharide (LPS) (a) or with LPS (b) and then analysed by RT‐PCR to detect inflammatory cytokines, including Tnfa, Ifng, Il4, Il6, Il12a and Il17a. Cytokine mRNA expression levels were normalized to GAPDH. Data are representative of five independent experiments. Error bars represent SD. *P < 0·05 (Student's t‐test).