Figure 5.

Suppression of T‐cell proliferation by M2‐polarized peritoneal macrophages. (a) CD4⁺ T cells isolated from the spleen of wild‐type mice were stimulated with anti‐CD3/CD28 beads and were either cultured alone (T cells only) or with untreated peritoneal macrophages (M0), interferon‐γ (IFN‐γ)‐treated peritoneal macrophages (M1), or interleukin‐4 (IL‐4)/IL‐13‐treated peritoneal macrophages (M2) at a ratio of 1 : 10 (macrophages: CD4⁺ T‐cells). Cells were collected after 4 days and analysed by the MTT assay. Bars show the mean ± SD from five separate cultures per condition.*P < 0·05 (Student's t‐test).