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. 2016 Oct 3;6:34307. doi: 10.1038/srep34307

Figure 1. Phylogentic analysis of GmDREBL and organic-specific gene expression.

Figure 1

(A) Phylogenetic tree analysis of GmDREBL protein and other DREB proteins. The phylogenetic tree was created with the high similarity sequences of AP2 domains from 6 G. max proteins and 11 Arabidopsis thaliana proteins using PHYLIP, and the bootstrap analyses were conducted with 200 replicates. The bootstrap value was showed in the branch and the length of each branch represents the distance between the sequences. (B) The organ-specific expression of GmDREBL in G. max cv. HN44. The roots, shoots and leaves of two weeks seedling were sampled. The pods with 2 cm length and the opening flowers of adult plant were sampled. Two developmental stages of soybean seeds were selected. Weight of a H1 developing seed is only 4% of highest seed fresh weight. Weight of a H5 developing seed is 35% of highest seed fresh weight. (C) The organ-specific expression of AtDREB2A and AtDREB2B in Arabidopsis thaliana. The homologues of GmDREBL with high identity in Arabidopsis were AtDREB2A and AtDREB2B (e-value < 1.3E-30). Their expression was evaluated by publicly available data of microarray in AtGenExpress of TAIR (http://www.arabidopsis.org).