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. 2016 Oct 3;6:34646. doi: 10.1038/srep34646

Figure 2. Flagellar trafficking of both Cop8 and ChR1 is affected in anterograde and retrograde IFT motor protein mutants.

Figure 2

(a,b) Cellular localization of Cop8 (green) in (a) and ChR1 (green) in (b), in fla8/Kinesin-2 mutant under permissive (22°C) and non-permissive temperature (33 °C). Incubation time at non-permissive temperature is mentioned at left side of the respective panel. For each representative image, DIC and merged images are also presented. (c,d) Quantitation of mean fluorescence intensity of Cop8 and ChR1 of the anterior spot signal are shown in (c and d), respectively. N = 200 cells counted per five independent experiments, for each conditions. Error bars represent standard deviation (s.d.). The significance (two-tailed t-test) is indicated, *P < 0.05, **P < 0.001. (e,f) Immunostaining of Cop8 in fla10/KHP1 mutant in e and in wild type cells in (f) (red) at 22 °C/dark and after 60 min of incubation at 33 °C/light. Merged images represent the accumulation of Cop8 at the anterior end, near the basal bodies. Scale bars represent 2 μm.