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. 2016 Oct 3;6:34702. doi: 10.1038/srep34702

Figure 2. Conditioned media from activated human macrophage cell lines and primary cells induce Bim-mediated apoptosis in target cells.

Figure 2

(A) THP-1 and U937 cells (human macrophage cell lines) were activated with LPS (100 ng/mL) and deoxynivalenol (DON) (200 ng/mL) and the conditioned medium was collected. The target cells (MEFs) were treated with this conditioned medium and apoptosis was measured after 48 h by annexin V-PI staining (left) or Bim induction was analysed by Western blot. Conditioned medium from activated RAW264.7 cells was used as positive control. (B) Human peripheral blood monocytes were differentiated into macrophages and were treated with LPS and DON. The conditioned medium was used to treat the target cells (MEFs). ER stress protein BiP and Bim induction was analysed by Western blot and apoptosis was measured by annexin V-PI staining after 48 h. Error bars +/− SEM; n = 3; unpaired, two-tailed Student’s t - test. *p < 0.005.