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. 2016 Oct 3;6:34702. doi: 10.1038/srep34702

Figure 3. Conditioned medium from activated macrophage cell lines induces ER stress target cells and this can be alleviated by chemical chaperones.

Figure 3

(A) MEFs were treated with the conditioned medium and XBP1 splicing was analysed at the indicated time points by digital droplet PCR (left) or by conventional PCR (right). Tunicamycin (200 ng/ml) was used as positive control. (B) MEFs were treated with the conditioned medium in the absence or in the presence of chemical chaperones TUDCA (1 mg/mL), compound C (1 μM), Genistein (5 μM) and the apoptosis response after 24 h was measured by annexin V-PI staining (left) and Bim/BiP protein modulation was assessed by Western blot (right). XBP1 splicing in MEFs treated with conditioned medium and TUDCA was assessed by PCR (bottom). The spliced and unspliced forms of Xbp-1 are indicated. Error bars +/− SEM; n = 3; unpaired, two-tailed Student’s t - test. *p < 0.05.