Table 1. Glycosyl composition of matrix polysaccharide in BY-2 cell wall materials.
Sugar amount (mol%) |
||||
---|---|---|---|---|
TFA-0 μM L.S. | TFA-1 μM L.S. | H2SO4-0 μM L.S. | H2SO4-1 μM L.S. | |
Arabinose | 28.5 ± 7.8 | 24.8 ± 3.6 | 0.0 | 0.0 |
Rhamnose | 5.6 ± 1.4 | 6.1 ± 1.1 | 0.0 | 0.0 |
Fucose | 0.0 | 0.0 | 0.0 | 0.0 |
Xylose | 15.2 ± 1.3 | 17.3 ± 1.6 | 0.0 | 0.0 |
Mannose | 6.1 ± 0.9 | 6.3 ± 0.4 | 4.5 ± 0.1 | 4.0 ± 0.5 |
Galactose | 22.4 ± 4.1 | 22.4 ± 2.6 | 0.5 ± 0.1 | 0.3 ± 0.2 |
Glucose | 13.0 ± 3.2 | 14.6 ± 1.8 | 93.1 ± 0.3 | 93.0 ± 0.4 |
Galacturonic acid | 8.2 ± 1.6 | 9.8 ± 1.6 | 1.9 ± 0.4 | 2.6 ± 0.2 |
Glucuronic acid | 1.0 ± 0.7 | 1.6 ± 0.2 | 0.0 | 0.0 |
Three technical replicates were performed. L.S., Lasalocid Sodium. TFA, TFA-soluble fraction; H2SO4, Hydrolyzed TFA-insoluble fraction with H2SO4.