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. 2016 Oct 3;6:32143. doi: 10.1038/srep32143

Figure 3. Effect of NAP on stomatal aperture, ABA level, and distribution of ABA and AhNCED1 in peanut in response to root stress.

Figure 3

(A) Stomatal aperture in leaves on treatment of roots with NAP for 2 h, followed by PEG treatment from 2 h up to 16 h. (B) ABA levels in leaves following the same treatment regime described for A. Bars are means (±SD) of n = 3. (C) AhNCED1 immunostaining of leaf, stem and root following the same treatment regime described for A. Control samples were treated with PBS; blue fluorescence (arrows) indicates the presence of AhNCED1; no color indicates a lack of staining. (D) ABA immunostaining of leaf, stem and root following the same treatment regime described for A. Control samples were treated with PBS; green fluorescence (arrows) indicates the presence of ABA; no color indicates a lack of staining. Bars = 50 μm for leaf panels and 100 μm for stem and root panels. (E) ABA distribution at the subcellular level in root of peanut following root stress. Red fluorescence indicates positive immunostaining; no color indicates a lack of staining. Bars = 100 μm.