Figure 1.

Stat5/Nf1-deficient bone marrow engrafts recipient animals. (A) Diagram depicting the breeding scheme to generate the five genetic backgrounds used in these studies. Stat5^ΔN/+/Nf1^Fcr/+ were generated and crossed with Stat5^ΔN/+/Nf1^Fcr/+/Mx1-Cre animals to generate the required genotypes. (B) Bone marrow was harvested from mice in each group and transplanted into syngeneic recipients. Four weeks after transplant and 2 weeks after induction of Cre recombinase, peripheral blood of recipient animals was immune-stained to measure the level of engraftment by Ly5.2⁺/Ly5.1⁻ donor cells. Recipient mice showed greater than 70% engraftment by donor cells. Typical results are shown. (C) Eight weeks after transplantation, DNA was extracted from peripheral blood nucleated cells of recipient animals. Polymerase chain reaction analysis was performed on genomic DNA from each animal to determine the degree of deletion of the floxed Nf1 allele. A band indicating deletion was detected in all animals from which adequate DNA was obtained. Typical results are shown for three animals. W: water; Δ: recombined flox allele.