Figure 2.
TRIB1 expression is decreased in human acute promyelocytic leukemia (APL) and does not correlate with MYC expression. (A) TRIB1 transcript levels in 21 cases of human APL are compared to levels in 440 cases of human non-APL acute myeloid leukemia (AML). Midlines represent median values, boxes represent 25th–75th percentiles, whiskers represent range of all values. TRIB1 levels are lower in APL than in non-APL AML (P<0.001). (Dataset reported values in log2 scale of GSE6891 have been transformed to linear values for clarity.) (B) TRIB1 values rise with maturation from human normal CD34 (n=5) to normal promyelocytes (n=5), but are low in human APL (n=14). Individual values are shown. [Intensity signal on arrays were scaled differently in the GSE6891 and GSE12662 datasets resulting in the different relative expression scales in (A) and (B)]. (C) Correlation plots of TRIB1 expression and MYC expression in GSE6891 for 461 AMLs, including APL, as well as for AMLs with isolated trisomy 8 (+8, n=20), AMLs with normal karyotype (NK, n=187), and APL alone (n=21). Trendlines are shown but R2 <0.5 for each of the comparisons. [Dataset log2 scale GSE6891 values were graphed for these comparisons; in contrast to (A), the values have not been linear transformed.]