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. 2016 Oct 3;4:20. doi: 10.1186/s40170-016-0159-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Blocking Wnt with dnLEF-1 reduces MCT-1 but not MCT-4 levels. qRT-PCR analysis was performed on RNA collected from SW480 (a) and SW620 (b) cells stably expressing dnLEF-1. Analysis was also performed for HCT116 cells (c) 72 h after lentiviral transduction of dnLEF-1 and DLD-1 dnLEF-1 cells (d) harvested 72 h after the addition of doxycycline. Graphs shown represent the average of three trials (+/− SEM). Whole cell lysates from each cell line (a–d) were harvested concurrently with RNA and were probed with the antibodies shown. (*p value <0.05; **p value < 0.01; ***p value < 0.001)