Fig. 6. H&E of non-degenerate (left) and degenerate (right) human IVDs (A). MCR-ALS calculated spectral profiles vs. reference spectral profiles of Factor 2 (950–1600 cm^–1, modelled with five factors) vs. collagen type I (BA), Factor 3 (950–1600 cm^–1, modelled with five factors) vs. collagen type II (BB), Factor 4 (950–1600 cm^–1, modelled with five factors) vs. collagen type II and elastin (BC), Factor 1 (950–1300 cm^–1, modelled with five factors) vs. elastin (BD), Factor 2 (950–1300 cm^–1, modelled with five factors) vs. chondroitin sulphate (BE) and Factor 5 (950–1300 cm^–1, modelled with five factors) vs. chondroitin sulphate (BF) as well as corresponding distribution maps (C) and distribution/tissue maps (D) of non-degenerate (left) and degenerate (right) human IVD sections.