Fig. 1. Effect of starting cell number on spheroid size and morphology. Spheroids were created from 500, 750, 1000, 1500, 2000 and 2500 C3A cells and cultured for 32 days. (A) Phase-contrast images of spheroids, images taken at day 7, 11, 14, 18, 21, 25, 28, 32. Scale bar = 100 μm. (B) Growth curve of spheroids over 32 days. Spheroid diameter (μm) was plotted against culture time (days). Data are represented as mean ± standard error (n = 3 in triplicate).
