Table 1. Toxicological analysis of spheroids. Spheroids were created from 1000 C3A cells by liquid-overlay technique, using 2D monolayer C3A cells as a comparison, and treated with 4 hepatotoxins. Cell viability was analysed IC50 values calculated. Data are represented as mean values (n = 3 in triplicate).
| Compound | IC50 value in C3A spheroids (μM) | IC50 value in C3A 2D monolayer (μM) |
| Paracetamol | 7212 | 33 826 |
| Diclofenac | 295 | Non toxic |
| Trovafloxacin | 65 | 440 |
| Fialuridine | 206 | 380 |