Table II. Percentage ratio of positive ion counts of stilbene glucosides to those of stilbene aglycons (Glu/Agl) in the authentic stilbene standards and specimens of Norway spruce phloem (middle of collapsed secondary phloem in tree JO) under frozen-hydrated and freeze-dried conditions.
To obtain the spectra of stilbene standards under frozen-hydrated conditions, their aqueous solutions were prepared with potassium chloride. Arithmetic means (and sd) for the standards are based on spectral data obtained from all of the standards of stilbene glucosides, while those for the phloem are based on spectra obtained from separate cell types (i.e., axial parenchyma, ray parenchyma, and intermediate areas composed mainly of collapsed sieve cells) isolated manually by the ROI tool in the data analysis software. P values in boldface are statistically significant (P < 0.05).
| Specimen | Glu/Agl |
F | P | |
|---|---|---|---|---|
| Frozen-Hydrated | Freeze-Dried | |||
| % | ||||
| Standards | ||||
| Piceid | 25 (7) | 8 (6) | 8.54 | 0.043 |
| Astringin | 40 (15) | 15 (12) | 5.16 | 0.086 |
| Isorhapontin | 52 (29) | 15 (12) | 4.29 | 0.107 |
| Phloem | ||||
| Piceid | 22 (7) | 50 (36) | 1.87 | 0.243 |
| Astringin | 45 (10) | 12 (11) | 15.62 | 0.017 |
| Isorhapontin | 75 (24) | 21 (16) | 10.56 | 0.031 |