Figure 4.
SVR9 and SVR9-LIKE genes in Arabidopsis. A, Gene models of three Arabidopsis genes coding for putative prokaryotic type IF3 proteins. Gene models were drawn as in Fig. 2A. Numbers of nucleotides of each exon excluding the lengths of untranslated regions were labeled on top of each exon. B, Transient expression of SVR9-GFP, SVR9L1-GFP, or GFP alone in wild-type Arabidopsis leaf protoplasts. A single representative protoplast was shown for each transformation. Merged images from GFP and chlorophyll channels are shown in the “Merge” lane. BF, Bright field. Bars, 10 μm. C and D, Tissue expression patterns of SVR9. Illustrated are GUS staining of 6-d-old (C) and 2-week-old (D) transgenic plants expressing the transcriptional fusion of PSVR9:GUS.