Figure 8.

Repression of chloroplast translation alters DR5:GUS expression pattern. Images of three representative cotyledons were shown for each genotype or treatment. A to C, Comparison of DR5:GUS expression patterns in wild-type, svr9-1, and svr9-1 svr9l1-1/+ backgrounds. DR5:GUS activities were assayed in cotyledons of 6-d-old seedlings grown on solid 1/2 MS medium. D to F, Effects of chloroplast translation inhibitors on DR5:GUS expression. Six-d-old wild-type background DR5:GUS seedlings maintained in liquid 1/2 MS culture were treated with 5 mm chloramphenicol, 5 mm spectinomycin, or equal amounts of ethanol (mock treatment) for 24 h before assaying DR5:GUS activities. Bars, 1 mm.