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. 2016 Aug 8;172(2):1167–1181. doi: 10.1104/pp.16.00183

Figure 4.

Figure 4.

Na+ and Cl− content analysis in the transgenic line E196A. In plants of E196, the point-mutated channel variant ALMT9E196A is expressed under the control of the native ALMT9 promoter in the genetic background of almt9-2. Na+ (A) and Cl− (B) measurements were performed in shoot and root tissue of hydroponically grown wt-2, almt9-2, and E196A plants upon treatment with control (0 mM) or NaCl (100 mM) solutions for 24 h. The results are shown as mean ± sd of n ≥ 5 biological replicates derived from two independent experiments. For statistical analysis, one-way ANOVA of each tissue and treatment and a Tukey-Kramer multiple comparison posttest was used. Different lowercase letters indicate significant differences in ion content (P < 0.05) under control conditions, capital letters under salinity. DW, Dry weight. C, Expression analysis of ALMT9 in wt-2 and E196A using qRT-PCR. ACT2 served as a reference gene. The expression levels were normalized to wt-2. Data are means ± sd from n = 2 biological replicates.