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. 2016 Aug 24;172(2):1273–1292. doi: 10.1104/pp.16.00501

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Figure 8. — Protein-protein interactions revealed from year two-hybrid assay. Y2HGold yeast strain with SH1 or CsCOP1 in the bait vector and CsHY5 and CsCOP1 in the prey vector were assayed for growth on double dropout (DDO; SD/-Trp/-Leu) or quadruple dropout (QDO; SD/-Leu-Trp-His-Ade) growth media. The positive control (pGBKT7-53 + pGADT7-T) and negative control (empty vectors pGBKT7-Lam + pGADT7-T) were included in the assay. Y2H assay revealed interactions between SH1 and CsHY5 as well as between CsCOP1 and CsHY5.