Table I. Genotyping and segregation analysis of self crosses in sec3a-1 mutant and complementation lines.
Shown is a summary of genotypes detected in Sd-resistant progeny of sec3a-1+/−, sec3a-1+/−/LAT52:SEC3a, and sec3a-1+/−/LAT52:GFP-SEC3a complementation lines, confirmed by PCR analysis. The χ2 test was applied for statistical analysis: P ≤ 0.005 indicates that observed data differ significantly from the expected data; NS, not significantly different.
| Genotype | N | sec3a-1+/− | sec3a-1−/− | χ2 | P |
|---|---|---|---|---|---|
| sec3a-1+/− (self-cross) | 72 | 72 | 0 | 36 | ≤0.0001 |
| sec3a-1+/−/LAT52:SEC3a line 1+/− | 8 | 5 | 3 | 0.063 | ≥0.80, NS |
| sec3a-1+/−/LAT52:SEC3a line 2+/− | 23 | 21 | 2 | 6.283 | ≥0.01 |
| sec3a-1+/−/LAT52:GFP-SEC3a line1+/− | 12 | 9 | 3 | 0.375 | ≥0.5, NS |
| Expected | 66.6% | 33.3% |