Table II. Genotyping and segregation analysis of reciprocal outcrosses in sec3a-1 mutant and complementation lines.
Shown is a summary of Sd-resistant and -sensitive progeny in reciprocal outcrosses of sec3a-1+/−, sec3a-1+/−/LAT52:SEC3a, and sec3a-1+/−/LAT52:GFP-SEC3a to the Columbia-0 (Col-0) background. *, The difference from expected could be explained by the variable expression levels of the complementation constructs in individual pollen. The χ2 test was applied for statistical analysis: P ≤ 0.005 indicates that observed data differ significantly from the expected data; NS, not significantly different.
| Reciprocal Outcrosses |
Sd Selection Marker |
||||
|---|---|---|---|---|---|
| N | Sensitive +/+ | Resistant sec3a-1+/− or sec3a-1−/− | χ2 | P | |
|
sec3a-1+/− (self-cross) |
1,140 |
52% |
48% |
323 |
≤0.0001 |
| Expected |
25% |
75% |
|||
| Pollen source: sec3a-1+/− |
408 |
100% |
0% |
408 |
≤0.0001 |
| Pollen recipient: Col-0 | |||||
| Pollen source: Col-0 |
483 |
50.5% |
49.5% |
0.033 |
≥0.8, NS |
| Pollen recipient: sec3a-1+/− | |||||
| Expected |
50% |
50% |
|||
| Pollen source: sec3a-1+/−/ |
228 |
72% |
28% |
2.8 |
≤0.1, NS |
|
LAT52:SEC3a line1+/− | |||||
| Pollen recipient: Col-0 | |||||
| Pollen source: sec3a-1+/−/ |
198 |
80% |
20% |
15.3 |
≤0.001* |
|
LAT52:GFP-SEC3a line 1+/− | |||||
| Pollen recipient: Col-0 | |||||
| Pollen source: sec3a-1+/−/ |
145 |
74% |
26% |
2.027 |
≥0.1, NS |
|
LAT52:SEC3a KRKR/A line 3+/− | |||||
| Pollen recipient: Col-0 | |||||
| Pollen source: sec3a-1+/−/ |
93 |
68% |
32% |
0.048 |
≤0.5, NS |
|
LAT52:GFP-SEC3a KRKR/A line 4+/− | |||||
| Pollen recipient: Col-0 | |||||
| Pollen source: sec3a-1+/−/ |
182 |
78% |
22% |
10.571 |
≤0.001* |
|
LAT52:SEC3a-ΔN line 2+/− | |||||
| Pollen recipient: Col-0 | |||||
| Pollen source: sec3a-1+/−/ |
127 |
87% |
13% |
22.763 |
≤0.001* |
|
LAT52:GFP-SEC3a-ΔN line 3+/− | |||||
| Pollen recipient: Col-0 | |||||
| Expected |
66.6% |
33.3% |
|||
| Pollen source: Col-0 |
188 |
52% |
48% |
0.340 |
>0.5, NS |
| Pollen recipient: sec3a-1+/−/ | |||||
|
LAT52:SEC3a line2+/− | |||||
| Pollen source: Col-0 |
112 |
51% |
49% |
0.036 |
>0.8, NS |
| Pollen recipient: sec3a-1+/−/ | |||||
|
LAT52:GFP-SEC3a line1+/− | |||||
| Expected | 50% | 50% | |||