Figure 1.

Polar exocytosis of NtPPME1 in the pollen tube tip. A, Characterization of NtPPME1 antibodies with total protein from 2-h germinated tobacco pollen tubes. The asterisk indicates the expected size of NtPPME1. M, Molecular mass in kilodaltons. B, Laser scanning of confocal microscopy (LSCM) image of a growing tobacco pollen tube expressing NtPPME1-GFP. C, Immunofluorescence labeling of NtPPME1 in tobacco pollen tube by using NtPPME1 antibodies. Scale bar = 25 µm. D, A representative time-lapse image series of expressing NtPPME1-GFP during pollen tube tip expansion. The fluorescent signals of NtPPME1-GFP remained tip-focused along with some intracellular punctate dots in the growing pollen tube. Scale bar = 25 µm.