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. 2016 Aug 16;172(2):1003–1018. doi: 10.1104/pp.16.00754

Figure 3.

Figure 3.

Polarity-associated NtPPME1 highlights cell plate during cytokinesis and is insensitive to BFA, ConcA, and wortmannin in tobacco BY2 cells. A and B, NtPPME1-GFP is polarity-associated and highlights the forming cell plate during cytokinesis in transgenic tobacco BY2 cells but degraded in the vacuole of a nondividing cell without polarity (B). C, Transgenic tobacco BY2 cells expressing both NtPPME1-GFP and GFP-AtSCAMP3 highlight cell plate during cytokinesis. D to F, Distinct responses of transgenic tobacco BY2 cells expressing NtPPME1-GFP and GFP-AtSCAMP3 to 10 μg mL−1 BFA, 2 μm ConcA, or 8.25 μm wortmannin 30-min treatment. Scale bar = 50 µm. G and H, Super resolution imaging by using STORM. BY2 cells were fixed and double-immunolabeled with NtPPME1 and OsSCAMP1 or AtEMP12 antibodies, respectively. Scale bar = 400 nm.