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. 2016 Aug 16;172(2):1003–1018. doi: 10.1104/pp.16.00754

Figure 5.

Figure 5.

NtPPME1-defined GDSVs, independent from TGN and CCVs, contribute to pollen tube tip growth and cell plate formation in tobacco BY2 cells. High-pressure frozen/freeze-substituted samples of 2-h germinated pollen tubes and synchronized wild-type BY2 cells were used for immunolabeling. A, Pollen tubes were immunolabeled with NtPPME1 antibodies. (1) Overview of the ultra-structure of a tobacco pollen tube tip. (2) NtPPME1-labeled exocytic secretory vesicles accumulated in the pollen tube tip region. (3) Ultrastructure of secretory vesicle which is directly derived from Golgi apparatus and is distinct from the TGN. (4) NtPPME1 labeled the GDSVs near the Golgi apparatus. B, ImmunoEM of synchronized BY2 cells. (1) NtPPME1 antibodies specifically labeled GDSVs (arrows), but not TGN or TGN-derived CCVs. (2) NtPPME1 antibody labeled the cell plate in BY2 cells (arrows). (3) BY2 cells were double-labeled with NtPPME1 and TGN marker OsSCAMP1 antibodies. NtPPME1-labeled GDSVs (10 nm, indicated by arrows) were distinct from TGN, which was labeled by OsSCAMP1 antibody (15 nm, indicated by arrowheads). (4) NtPPME1 antibody labeled secretion vesicles on cell plate (10 nm, arrows) were separate from CHC antibody-labeled CCVs on or near the cell plate (6 nm, arrowheads). 5 and 6 are enlarged areas from 4.