Table I. Distribution of immunogold labeling with antibodies against NtPPME1 and OsSCAMP1 of tobacco pollen tubes.
To elucidate the distribution of the NtPPME1 antigens in GDSV, which is distinct from OsSCAMP1 antigen in the TGN, photographs of GDSV with distinguishable TGN, Golgi apparatus, endoplasmic reticulum (ER), and MVB were taken and the number of gold particles were counted for each individual organelle. Fifty-three GDSVs from different fixations of tobacco pollen tubes were evaluated for the label. Gp, Number of gold particles.
| Antibody | GDSV | Tip Vesicle | TGN | Golgi | ER | MVB | Gp (%) |
|---|---|---|---|---|---|---|---|
| NtPPME1 | 175(38.7) | 227 (50.3) | 16 (3.6) | 15 (3.1) | 12 (2.6) | 7 (1.7) | 452 (100) |
| OsSCAMP1 | 5 (0.7) | 298 (47) | 217 (42.8) | 20 (3.2) | 17 (2.7) | 22 (3.5) | 579 (100) |