Table II. Distribution of immunogold labeling with antibodies against NtPPME1, OsSCAMP1, CHC, VSR, and AtEMP12 in BY2 cells.
To elucidate the distribution of the NtPPME1 antigens in the GDSV, photographs of GDSV with distinguishable TGN, CCV, Golgi apparatus, and MVB were taken and the number of gold particles was counted for each individual organelle. Forty-four GDSVs from different fixations of BY2 cells were evaluated for the label. Gp, Number of gold particles; CHC, Clathrin H chain.
| Antibody | GDSV | TGN | CCV | CP | Golgi | MVB | Gp (%) |
|---|---|---|---|---|---|---|---|
| NtPPME1 | 139 (38.2) | 13 (3.6) | 8 (2.2) | 171 (47.0) | 24 (6.6) | 9 (2.5) | 364 (100) |
| OsCAMP1 | 14 (3.6) | 110 (27.9) | 98 (24.9) | 127 (32.2) | 31 (7.9) | 14 (3.6) | 394 (100) |
| CHC | 3 (1.7) | 63 (35.2) | 90 (50.3) | 15 (25.7) | 3 (1.68) | 5 (2.79) | 179 (100) |
| VSR | 1 (1.5) | 2 (3.0) | 1 (1.5) | 3 (4.5) | 3 (4.5) | 56 (84.8) | 66 (100) |
| AtEMP12 | 2 (3.2) | 6 (9.5) | 1 (1.6) | 4 (6.3) | 44 (69.8) | 6 (9.5) | 63 (100) |