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OsRAD1 is essential for meiosis. A, Chromosome spreads of male meiocytes in wild type (top row) and Osrad1 (bottom row). The abnormal chromosome associations in Osrad1 are pointed out with arrows. B, Homologous bivalents are formed in Osrad1. FISH analysis of the chromosome interaction in wild type and Osrad1. C, Immunostaining for ZEP1 (green) in wild type and Osrad1 at pachytene. OsREC8 signals (red) were used to indicate the meiocytes. Bars = 5 µm.
