Figure 3.

Decoupling between early oxidation and subsequent induction of cell death under chronic iron limitation. Scatter plots of degree roGFP oxidation in P. tricornutum cells targeted to the nucleus (3 h post H₂O₂ treatment, circles) and mitochondria (0.5 h post H₂O₂ treatment, triangles) in response to 0 to 150 µM H₂O₂, and level of cell death measured by Sytox positive cells 24 h posttreatment. A, Replete. B, Phase I (days 1–4 of iron limitation). C, Phase II (since day 5 of iron limitation). Each graph presents at least 130 samples.