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. 2016 Aug 24;172(2):1061–1073. doi: 10.1104/pp.16.01026

Figure 2.

Figure 2.

GPI modification is required for the targeting of BG_pap and PDCB1. Localization is shown for BG_pap and PDCB1 in transient expression in N. benthamiana. A to B′′, Localization of SP-mCitrine fused to full-length BG_pap (mCit-BG). C to D′′, Localization of SP-mCitrine fused to full-length PDCB1 (mCit-CB). E and G, Localization of mCit-BG (E) and mCit-CB (G) in the presence of the GPI biosynthesis inhibitor mannosamine. F to F′′, Localization of SP-mCitrine fused to BG_pap in which the C-terminal part is deleted (mCit-BGΔC). H to H′′, Localization of SP-mCitrine fused to PDCB1 in which the C-terminal part is deleted (mCit-CBΔC). B to B′′ and D to D′′ show localization in plasmolyzed cells stained with FM4-64. F to F′′ and H to H′′ show colocalization with the ER marker mCherry-ER. Arrows in A and C indicate peripheral puncta. In plasmolyzed cells, the dashed white line marks the cell wall, asterisks indicate the apoplastic space formed by the receding protoplast, and triangles indicate the position of the PM. Images in E to H′′ are Z projections of several confocal slices. Bars = 10 µm.