Figure 5.

Ectodomain in BG_pap and PDCB1 enhances Pd accumulation. Pd and PM accumulation levels are shown for the full-length fusions compared with those of GPI-anchored mCitrine in transient expression in N. benthamiana. A and C, Representative confocal images of cells expressing mCit-GPI^BG and mCit-BG (A) or mCit-GPI^CB and mCit-CB (C). B and D, Relative fluorescence intensities quantified for each fusion protein at two distinct regions as indicated in A, PM (yellow circle) and Pd (red circle). Values represent mean fluorescence intensity at PM or Pd relative to mean fluorescence intensity at PM measured for mCit-GPI^BG (B) and mCit-GPI^CB (D). The white dashed lines in B and D represent the reference line corresponding to 1. Error bars represent se. Lowercase letters indicate statistically significant differences determined by nonparametric Kruskal-Wallis one-way ANOVA test for multiple groups (***P < 0.001; ****P < 0.0001; ns=not significant). The experiment was performed two times with similar results. Bars = 10 μm.