Figure 2.

NRT1.1-GFP accumulation is differentially regulated by N in root tissues. Fluorescence quantification in different root tissues of 8-d-old plants form pNRT1.1::NRT1.1-GFPLoop16-3 transgenic line expressing NRT1.1-GFP protein fusion in the absence or presence of NO₃⁻ (1 mm) or 0.5 mm Gln. Fluorescence was quantified respectively in primary root tip, cortex, and epidermis of primary root (above the first emerged primordium), unemerged primordia stage IV to VI, emerged primordia, and growing LRs (±0.5 mm). Values are the mean of eight to 12 plants from two independent experiments and are normalized to Col (error bars are se). For each tissue, data were analyzed through one-way ANOVA, followed by a Tukey’s test as a post hoc analysis, and are statistically significant at P < 0.05.