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. 2016 Aug 22;172(2):1154–1166. doi: 10.1104/pp.16.01088

Figure 6.

Figure 6.

Phenotypes of sporophytes formed on ΔPpXLG by crossing with Vx:DsRed. A, Dissected sporophytes of Vx:dsRED × GdWT and Vx:dsRED × Gd:ΔPpXLG crosses showing stomata (white arrows) and spores. B, Germination efficiency determined by 9 replicates of approximately 100 spores. The average values are plotted as gray diamonds. Total mean values are indicated as boxes. C, Segregation of genetic marker as determined via PCR or dsRED fluorescence microscopy. Mean and SD of 5 replicates each with 30 descendants are shown. D, Top view of P. patens plants wild type (WT) Gd, Gd:ΔPpXLG, and a representative recombinant cross Vx:dsRED × Gd:ΔPpXLG grown on reduced nitrogen media. WT plants form sporophytes, whereas no sporophytes were formed on either Gd:ΔPpXLG or Vx:ΔPpXLG.