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. 2016 Aug 10;172(2):1074–1088. doi: 10.1104/pp.16.01097

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Figure 5. — Effects of DTT and the ROS scavengers AsA and DMTU on Pro accumulation and PDH1_pro:LUC2 activity. A, Effects of DTT treatment on the Pro accumulation of seedlings at high ψ_w. FW, Fresh weight. Data are means ± se (n = 10–44) combined from three experiments. Significant differences (P ≥ 0.05) compared with the wild type in the same treatment or between 0 and 5 mm DTT treatment of the wild type are marked with asterisks. B, Effects of DTT treatment at −1.2 MPa on Pro accumulation. Data are means ± se (n = 10–44) combined from three experiments. Significant differences (P ≥ 0.05) compared with the wild type are marked with asterisks. C, Effects of AsA and DMTU on Pro levels at −0.25 and −1.2 MPa. Data are means ± se (n = 10–44) combined from three experiments. Significant differences (P ≥ 0.05) compared with the mock treatment are marked with asterisks. D, Effects of AsA on PDH1_pro:LUC2 activity in the wild type, 4442-4, and 4255-1 at high ψ_w (−0.25 MPa) or low ψ_w (−1.2 MPa). Data are means ± se (n = 10–44) combined from three experiments. Significant differences (P ≥ 0.05) compared with the mock treatment are marked with asterisks. E, Effects of DMTU on PDH1_pro:LUC2 activity in the wild type, 4442-4, and 4255-1. Data are means ± se (n = 10–44) combined from three experiments. Significant differences (P ≥ 0.05) compared with the mock treatment are marked with asterisks. Luminescence intensities in D and E are given in photons (p) per second (s) per cm² per steradian (sr). F, False-color imaging of PDH1_pro:LUC2 activity in representative seedlings from the experiments reported in E.