Figure 3.

A, In gel activity assays of mitochondrial proteins using d-lactate or glycolate as substrate. Mitochondria isolated from Arabidopsis leaves (leaf) as well as from cell suspension culture (cell culture) were solubilized with 5% (w/v) digitonin and subsequently separated on a BN gel. In gel activity assays were performed using 10 mm d-lactate or 10 mm glycolate as substrate. The position of the activity signal is marked on the left with the red arrow. Positions of membrane-bound protein complexes are given on the left (I, complex I; III₂, dimeric complex III; V, ATP synthase complex; I + III₂, supercomplex formed by complex I and dimeric complex III). B, d-LDH peptides identified on the BN gel by mass spectrometry. ^aAccession numbers of identified proteins as given by The Arabidopsis Information Resource (http://www.arabidopsis.org/). ^bName of the identified protein. ^cCalculated molecular mass of the identified proteins as deduced from the corresponding gene. ^dProbability score for the protein identifications based on mass spectrometry analysis and MASCOT search. ^eNumber of unique peptides. ^fSequence coverage of the protein by identified peptides.