Fig. 1.

IAA oxidation in Arabidopsis is regulated by the dioxygenase gene family member At1g14130 (AtDAO1). (A) IAA feeding experiment. Formation of labeled IAA conjugates and catabolites after incubation of 7-d-old Arabidopsis Col-0, dao1-1, and dao1-2D seedlings with [¹³C₆]IAA. Samples were analyzed in three independent biological replicates. Error bars represent SD. FW, fresh weight. (B) Structure of the Arabidopsis DAO1 gene with indication of the position of the T-DNA insertions (triangles) in the different dao1 alleles studied.