Fig. S1.

Phylogenetic relationships between AtDAOs and related proteins. (A) Phylogenetic tree of the AtDAO1 and AtDAO2 and related Arabidopsis proteins. Arabidopsis 2-ODD sequences were aligned using MUSCLE within Geneious and trimmed to remove unaligned residues. Phylogenetic analysis was carried out using PhyML and MrBayes within ToPALI (www.topali.org/). A section of the MrBayes tree was selected and displayed in MEGA6. Numbers show branch support values; the scale bar shows substitutions per site. (B) Sequence alignment of the active site region of AtDAOs and related proteins from various plant species identified at https://phytozome.jgi.doe.gov/pz/portal.html. Sequences were aligned using MUSCLE. The Fe binding residues are indicated with red dots, and the 2-oxoglutarate binding residues are indicated with blue dots. Residue numbering is from the AtDAO1 (At1g14130) sequence.