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. 2016 Sep 12;113(39):10872–10877. doi: 10.1073/pnas.1606360113

Fig. S3.

Fig. S3.

Site-specific 4-DMN labeling of HdeA and HdeB for monitoring local conformational changes. (A) The molecular structure of alk-4-DMN. (B) Fluorescence gel and corresponding Coomassie blue-stained SDS/PAGE of 4-DMN–labeled proteins. Purified azide-containing proteins at specific sites were labeled with alk-4-DMN by Cu(I)-catalyzed azide-alkyne cycloaddition reaction (CuAAC). A WT, HdeA-WT; A35, HdeA-35LAPK; A35 (D20A), HdeA-D20A/35LAPK; B24, HdeB-24LAPK. (C) Schematic model for the conformational changes of HdeA and HdeB with the corresponding fluorescence change at pH 7 and pH 2. (D) The fluorescence intensity of 4-DMN labeled proteins measured at pH 7 (black line) and pH 2 (red line).