The γ1 subunit supports membrane expression of CaV1.1 in tsA201 cells. (A) Representative peak calcium current (test potential: +30 mV) and peak I-V relationships in tsA201 cells transfected with YFP-CaV1.1, β1a, and α2-δ1 and γ1. Calibrations: 1 pA/pF (vertical), 50 ms (horizontal). (B) Representative charge movements (Vtest of −20, 0, +20, and +40 mV) and average Q-V relationships in tsA201 cells transfected with YFP-CaV1.1, β1a, α2-δ1 and γ1. Calibrations: 2 pA/pF (vertical), 10 ms (horizontal). For comparison, the smooth curves fitted to the peak I-V and Q-V data for tsA201 cells transfected with YFP-CaV1.1, β1a, α2-δ1 and WT Stac3 (Fig. 1 A and B) are replotted in blue.