Table S1.
Charge movement and conductance fit parameters
| Cell-type/constructs | Q-V Qmax, nC/µF | I-V Gmax, nS/nF | ||
| VQ, mV | kQ, mV | V1/2, mV | kG, mV | |
| Dysgenic myotubes + CaV1.1 | 7.7 ± 0.7 (10) | 170.0 ± 16.6 | ||
| −15.7 ± 0.6 | −15.7 ± 0.6 | 27.9 ± 1.9 | 7.1 ± 0.2 | |
| tsAs CaV1.1, α2-δ1, β1a + Stac3 | 12.3 ± 0.6 (10) | 252.7 ± 14.5 | ||
| −4.4 ± 1.2 | −4.4 ± 1.2 | 30.1 ± 0.7 | 8.5 ± 0.3 | |
| tsAs CaV1.1, α2-δ1, β1a + Stac3 + γ1 | 7.3 ± 0.5 (11) | 131.2 ± 5.1 | ||
| −13.0 ± 0.7 | −13.0 ± 0.7 | 26.1 ± 0.8 | 9.5 ± 0.4 | |
| tsAs CaV1.1, α2-δ1, β1a + γ1 | 6.9 ± 0.7 (9) | ND | ||
| −4.9 ± 0.9 | 20.3 ± 0.7 | |||
| Dyspedic myotubes | 4.2 ± 0.4 (7) | 42.3 ± 5.1 | ||
| 9.0 ± 1.5 | 9.0 ± 1.5 | 29.0 ± 0.5 | 7.8 ± 0.3 | |
| Stac3 KO myotubes | 3.7 ± 0.3 (8) | 52.8 ± 3.1 | ||
| −16.2 ± 0.8 | −16.2 ± 0.8 | 14.5 ± 0.7 | 9.0 ± 0.4 | |
| Stac3 heterozygous myotubes | 7.9 ± 0.5 (9) | 212.0 ± 15.0 | ||
| −4.5 ± 0.5 | −4.5 ± 0.5 | 16.5 ± 0.3 | 6.6 ± 0.1 | |
| Stac3 KO myotubes + Stac3 | 7.3 ± 0.2 (12) | 193.8 ± 11.3 | ||
| −5.9 ± 0.4 | −5.9 ± 0.4 | 13.8 ± 0.4 | 6.2 ± 0.3 | |
| Stac3 KO myotubes + Stac3W280S | 5.6 ± 0.4 (7) | 107.2 ± 7.1 | ||
| −19.2 ± 0.7 | −19.2 ± 0.7 | 13.1 ± 0.4 | 7.3 ± 0.2 | |
| tsAs CaV1.1, α2-δ1, β1a + Stac3W280S | 4.4 ± 0.5 (7) | 63.4 ± 3.9 | ||
| −0.4 ± 0.6 | 17.3 ± 0.5 | 32.2 ± 1.1 | 11.7 ± 0.5 | |
Average values of parameters obtained by fits of Eqs. 1 and 2 to I-V and Q-V data, respectively (Materials and Methods). Data are given as mean ± SEM, with the numbers in parentheses indicating the number of cells tested. The average parameters listed above were used to produce the smooth curves through the Q-V and peak I-V data shown in Figs. 1, 3, 4, and 6 and Fig. S2. ND, not determined.