Table 1.
Peak | Carotenoid | tR (min) | λmax (nm) | [M+H]+* | Main product ions† |
1 (L, E+n-l)‡ | Lutein | 15.7 | 420, 444, 472 | 569.4335 (−3.1/28.8) | 551.4281 [M+H-18]+; 495.3642 |
2 (E+n-l) | Dilauroyl-5,6-epoxy-lutein | 33.8 | 418, 443, 469 | 949.7668 (2.6/42.3) | 931.7601 [M+H-H2O]+; 749.5791 [M+H-FA]+ |
549.4184 [M+H-2FA]+ | |||||
3 (E+n-l) | Unknown¶ | 35.3 | 420, 444, 472 | — | — |
4 (E+n-l) | Lauroyl-myristoyl-5,6-epoxy-lutein | 36.2 | 418, 443, 469 | 977.7970 (−1.4/39.2) | 959.7912 [M+H-H2O]+; 777.6150 [M+H-FA]+ |
749.5890 [M+H-FA]+; 549.4179 [M+H-2FA]+ | |||||
5 (E+n-l) | Myristoyl-oleyl-5,6-epoxy-lutein | 37.1 | 418, 443, 469 | 1,059.8695 (−2.9/45.6) | 1,041.8592 [M+H-H2O]+; 831.6697 [M+H-FA]+ |
777.6196 [M+H-FA]+;549.4162 [M+H-2FA]+ | |||||
6 (E+n-l) | Lauroyl-linoleoyl-lutein | 45.5 | 420, 444, 472 | 1,013.8339 (−1.96/29.3) | 995.8237 [M+H-H2O]+; 813.6589 [M+H-FA]+ |
733.5929 [M+H-FA]+; 533.4187 [M+H-2FA]+ | |||||
7 (E+n-l) | Palmitoyl-linoleoyl-lutein§ | 46.3 | 420, 444, 472 | 1,067.8813 (1.74/30.2) | 1,049.9804 [M+H-H2O]+; 811.6524 [M+H-FA]+ |
789.6613 [M+H-FA]+; 533.4249 [M+H-2FA]+ | |||||
8 (E+n-l) | Palmitoyl-linoleoyl-lutein§ | 47.0 | 420, 444, 472 | 1,067.8859 (1.84/29.5) | 1,049.9797 [M+H-H2O]+; 811.6537 [M+H-FA]+ |
789.6595 [M+H-FA]+; 533.4262 [M+H-2FA]+ | |||||
9 (E+n-l) | Palmitoyl-linoleoyl-lutein§ | 47.5 | 420, 444, 472 | 1,067.8806 (1.06/32.8) | 1,049.9992 [M+H-H2O]+; 811.6529 [M+H-FA]+ |
789.6604 [M+H-FA]+; 533.4236 [M+H-2FA]+ | |||||
10 (E+n-l) | Oleyl-linoleoyl-lutein | 48.4 | 420, 444, 472 | 1,095.9183 (1.35/19.8) | 1,077.9015 [M+H-H2O]+; 813.6593 [M+H-FA]+ |
815.6758 [M+H-FA]+;533.4278 [M+H-2FA]+ |
m/z experimental value of the molecular ion. Numbers in parenthesis are mass error in ppm and SigmaFit value.
Main products ion arising from MS2-based reactions.
Peak present in liver extract (L) and/or in ears+nose-leaf (E+n-l) extract.
Peak not identified.
Trans and cis isomers.