Fig. S7.

HA-specific Th1 cells do not potentiate the cytotoxic effect of CTLs. (A and B) Enumeration of orexin⁺ (A) and MCH⁺ (B) neurons 60 d after transfer of either CTLs alone, concomitant transfer of CTLs and Th1, or transfer of CTLs and Th1 15 d apart in Orex-HA (red squares) or WT (blue circles) mice. The type of transferred cells (and timing of injection) is indicated in the bottom of the graph. The group of Orex-HA mice receiving only CTLs is identical to that shown in Fig. 4. Results are expressed as mean ± SEM of 6–13 mice per group. Statistical analyses were performed by using one-way ANOVA, P values were adjusted for multiple comparisons by using Sidak’s test. ns, not significant. (C) Enumeration of cataplexy episodes and sleep attacks during 24 h in WT mice (blue circles) and Orex-HA animals (red squares) after concomitant transfer of CTLs and Th1 cells. Results are expressed as mean ± SEM of six mice per group from two independent experiments.