Table S2.
Primers and vectors used in this study
| Primer | Sequence (5′-3′) | Restriction endonuclease | Application and vector |
| tLGP2-qF | GTCTCCCAAGAACCTCTG | Analytical real-time qRT-PCR | |
| tLGP2-qR | GGACCACTTTTTGGCTTG | ||
| tMDA5-qF | TTACAGGGCTCAACCATC | Analytical qRT-PCR | |
| tMDA5-qR | CATGCTTGACCACATTTG | ||
| tLGP2-5′RACE | GCCGTGTCCTCCGCAGTTTGTGCTCTAGGT | 5′ RACE | |
| tLGP2-5′RACE | GTTGTAGGTGGTGTCCTTGTGCGTGTGGTG | 5′ RACE nested | |
| tLGP2-3′RACE | GGGCCAGTCAGAGCGTGTACTCGTTTGT | 3′ RACE | |
| tLGP2-3′RACE | CTGCGGCGGGAGCTGACCAATGAG | 3′ RACE nested | |
| tMDA5-5′RACE | GCACAGGCTCCACCTGGATATATGT | 5′ RACE | |
| tMDA5-3′RACE | CAAGCATGGGGAACGATGATGGTGC | 3′ RACE | |
| tLGP2-BamH-F | CGGGATCCATGGAGCTGCGGCCCTAC | BamHI | PCR for constructing tLGP2-3Tag-8 using pCMV-3Tag-8 vector |
| tLGP2-Xho-R | CCGCTCGAGGTCCCCGGAGAGGTTGG | XhoI | |
| tMDA5-BamH-F | CGGGATCCATGTCGAATGGGCATTCCT | BamHI | PCR for constructing tMDA5-3Tag-8 using pCMV-3Tag-8 vector |
| tMDA5-Xho-R | CCGCTCGAGATCCTCATCACTAAACAAAC | XhoI | |
| tMITA-BspE-F | CGGTCCGGAATGCCCCACTCCAGCCTGC | BspEI | PCR for constructing tMITA-FL-myc using pCS-myc-N vector |
| tMITA-Xho-R2 | CCGCTCGAGTCAGAAGACATCCGTGCGG | XhoI | |
| tMDA5-K188Q-F | TTGAGGGTTCTTCAACAAACAGGAAACGAT | PCR for constructing mutant tMDA5 K188Q using pCMV-3Tag-8 vector | |
| tMDA5-K188Q-R | ATCGTTTCCTGTTTGTTGAAGAACCCTCAA | ||
| tMDA5-A402K-F | GAAGTTGTTAAATCCTGTGATGTTATCATC | PCR for constructing mutant tMDA5 A402K using pCMV-3Tag-8 vector | |
| tMDA5-A402K-R | GATGATAACATCACAGGATTTAACAACTTC | ||
| hMDA5-Q187K-F | ATGTTCTTCGTAAAACAGGAAACAATGAAC | PCR for constructing mutant hMDA5 Q187K based in hMDA5 expression vector | |
| hMDA5-Q187K-R | GTTCATTGTTTCCTGTTTTACGAAGAACAT | ||
| hMDA5-K405A-F | GAAGTTGTCGCGTCCTGTGATATTATTATC | PCR for constructing mutant hMDA5 K405A based in hMDA5 expression vector | |
| hMDA5-K405A-R | GATAATAATATCACAGGACGCGACAACTTC | ||
| tIFN-β-pro.-Kpn-F1 | GGGGTACCTTTGCTTTCCTTTGCTTTG | KpnI | PCR for constructing pGL3-tIFN-β-promoter using pGL3-Basic vector |
| tIFN-β-pro.-Nhe-R | GGAATTCGCTAGCCCTTCCTCCATGGGCATGG | NheI | |
| 6964-fwd1 | CCCCAGCYRCCGCTAGTTGCA | PCR for amplify the tRIG-I genomic region which contains the start codon ATG | |
| 8733-low1 | AAGGCTCCTCAAACTCTG | ||
| RIG-I F | CAGACAAGGAGAACTGGCC | PCR amplification to capture the RIG-I gene using human, flying lemur and tree shrew cDNAs as the template | |
| RIG-I R2 | CTGGAATCTCAAATGTCTTG |
*
Restriction endonuclease sites introduced by PCR are underlined.