Fig. S4.

Expression of GIV in various HeLa cell lines used in this study. Equal aliquots of whole-cell lysates of control (shControl), GIV-depleted (shGIV), and GIV-depleted HeLa cells stably expressing GIV-WT, GIV-DD, and GIV-FA constructs that are insensitive to the action of shRNA were analyzed for GIV and tubulin by immunoblotting. The efficacy of GIV depletion by shRNA was analyzed by band densitometry using LI-COR Odyssey and was determined to be >90% compared with control cell lysates. Both GIV-WT and mutants were expressed equally well and at levels similar to endogenous GIV.