Skip to main content
. 2016 Sep 13;113(39):11046–11051. doi: 10.1073/pnas.1612826113

Fig. S5.

Fig. S5.

Additional metrics to evaluate the performance of MERFISH measurements. (A) The total number of RNAs decoded without (Exact) and with (Corrected) error correction. (B) The confidence ratio for all barcodes representing real RNAs (blue) and the blank controls (red) sorted from largest to smallest value. The confidence ratio for any given gene (or barcode) is defined as the ratio between the number of exact matches to this barcode and the total number of exact matches to this barcode plus matches with single-bit errors. Of the 130 barcodes encoding real RNAs, 123 have a confidence ratio larger than that of the largest confidence ratio of the blank barcodes. (C) The error rate (the fraction of measured barcodes that contain a given bit flip) for each bit. Both 1-to-0 error rates (blue) and 0-to-1 error rates (red) are shown for each bit. The data presented in this figure represent the error properties of the dataset presented in Fig. 3 and are representative of those observed for all other datasets.