Fig. S2.

Biologically active TAMRA-pep1. (A) Measurement of the pH of Arabidopsis cell suspensions after 20 min of exposure to different concentrations of pep1 or TAMRA-pep1 (n = 9). (B) Total luminescence of aquorin-expressing Arabidopsis plants subjected to the aquaporin Ca²⁺ assay and treated with 10 nM pep1 or TAMRA-pep1 (n = 9). (C) Root growth inhibition of Col-0 in the presence of pep1 or TAMRA-pep1 (n = 10). (D) RT-PCR analysis of PEPR1 gene in 6-d-old Arabidopsis seedlings treated for 60 min with 100 nM pep1 or TAMRA-pep1. GAPDH (At1g13440) and water served as loading control and control, respectively. These experiments were repeated at least twice, with similar results. Error bars indicate SD. n, number of wells in A and number of seedlings in B and C.