Fig. S5.

AtPep1 and PEPR1 internalize with similar dynamics. (A) Internalization of TAMRA-pep1 in root epidermis of 5-d-old Arabidopsis seedlings after incubation with TAMRA-pep1 (100 nM, 10 s), three washouts, and imaging at the indicated time points. Root meristem epidermal cells are shown. (B) PEPR1-GFP internalization was triggered by pep1 in a dose-dependent manner. Seedlings were treated with different concentrations of pep1 for 10 s, washed three times, and imaged after chases of 5 min and 40 min. (C) Internalization of PEPR1-GFP after treatment with pep1 (100 nM, 10 s) as in A. Application of flg22 and mock (water) did not change the PEPR1-GFP localization. (D) PEPR1-GFP internalization on constant elicitation with pep1. The 5-d-old pepr1pepr2 seedlings complemented with PEPR1-GFP expressed from the RPS5A promoter were used in B, C, and D. (Scale bars: 10 µm.)