Cloning |
Genomic PEPR1 |
Forward |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCAATGAAGAATCTTGGGGGGTTGTTC |
Reverse |
GGGGACCACTTTGTACAAGAAAGCTGGGTACCGAACTGAATCAGAGGAGCA |
Genomic PEPR2 |
Forward |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCAATGAGGAATCTTGGGTTACTCG |
Reverse |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGTGAACTGAACCCGAAGTGCTTCT |
Promoter PEPR1 |
Forward |
GGGGACAACTTTGTATAGAAAAGTTGCTTCACTGATCTGTTTGTTGCAAAC |
Reverse |
GGGGACTGCTTTTTTGTACAAACTTGCCTGAGTTTAAAGATCGAGAAACATG |
Promoter PEPR2 |
Forward |
GGGGACAACTTTGTATAGAAAAGTTGCTATTAGGGTGGTCTATCGGTCAG |
Reverse |
GGGGACTGCTTTTTTGTACAAACTTGCATTAGAGCTCAAGAGACTGAAATATG |
CDS Auxilin2 |
Forward |
CACCATGGATGATTTCACAGGATTGTT |
|
Reverse |
TCAAAAGAGTTCCTCTGAGTTGAAT |
RT-PCR |
GAPDH
|
Forward |
TTGGTGACAACAGGTCAAGCA |
Reverse |
AAACTTGTCGCTCAATGCAA |
PEPR1
|
Forward |
GTTTTGGCTGAGGAAAGACG |
Reverse |
ACATTGTACCGTGCAGACCA |